Braxton - Week 6&& updates? - Dr. B
Braxton - week 5 updates? - Dr. B
Braxton - do you have updates for Week 4? - Dr. B
Week 4
Day 5 (6/23/14):
Week 3
Day 1 (6/17/14):
For our first day we learned how to calibrate micropipettors on an analytic balance and to evaluate the precision and accuracy of different measurements using P1000 P200 P20 micropipettors.
We calculated the values of standard deviation, percent error, and coefficient of variations and graphed them in order to show the accuracy and precision of each pipettor. From the data we discovered that the P20 was the most accurate, followed by the P1000. Although the P1000 is the most precise.
Day 2 (6/18/14):
We did the Buffers and Solutions lab in order to learn how to make solutions properly. In this lab we made seven solutions that required different calculation in order to make the proper solution. This lab allowed us to get used to the lab and learn where key items are in the lab and use different tools in the lab.
Day 3 (6/19/14):
First we finished the buffers and solutions lab form the previous day and then learned how to nano drop
Once we used the Nanodrop we learned how to send a plasmid off to DNA sequencing. We sent of a 10uM primer that we dilluted from a 100uM primer. After we dilluted the water and filled out the request we took our plasmid over to the ICMB building.
Day 4 (6/20/14):
We did primer dilution in order to send our plasmid off to get sequenced, and after that we started day one of the transformation efficiency with the DH5a.
Braxton - week 5 updates? - Dr. B
Braxton - do you have updates for Week 4? - Dr. B
Week 4
Day 5 (6/23/14):
Week 3
Day 1 (6/17/14):
For our first day we learned how to calibrate micropipettors on an analytic balance and to evaluate the precision and accuracy of different measurements using P1000 P200 P20 micropipettors.
We calculated the values of standard deviation, percent error, and coefficient of variations and graphed them in order to show the accuracy and precision of each pipettor. From the data we discovered that the P20 was the most accurate, followed by the P1000. Although the P1000 is the most precise.
Day 2 (6/18/14):
We did the Buffers and Solutions lab in order to learn how to make solutions properly. In this lab we made seven solutions that required different calculation in order to make the proper solution. This lab allowed us to get used to the lab and learn where key items are in the lab and use different tools in the lab.
Day 3 (6/19/14):
First we finished the buffers and solutions lab form the previous day and then learned how to nano drop
Once we used the Nanodrop we learned how to send a plasmid off to DNA sequencing. We sent of a 10uM primer that we dilluted from a 100uM primer. After we dilluted the water and filled out the request we took our plasmid over to the ICMB building.
Day 4 (6/20/14):
We did primer dilution in order to send our plasmid off to get sequenced, and after that we started day one of the transformation efficiency with the DH5a.